WGS of FSL A6-0213 - SRA

SRX18469715: WGS of FSL A6-0213
1 ILLUMINA (Illumina MiSeq) run: 913,329 spots, 262.4M bases, 153Mb downloads

Design: The genomic DNA was extracted by using QIAamp DNA Mini Kit (Qiagen, Valencia, CA) according to the manufacturers instruction, which was modified to include an additional 45 min lysis step. Briefly, cultures grown overnight in BHI broth at 32 C were pelleted by centrifugation and subsequently lysed with 20 L of proteinase K (20 mg/mL) by incubation for 45 min at 56C. DNA was eluted twice in 50-L volumes of 10 mM Tris-HCl (pH 8.0), and DNA purity was assessed using a Nanodrop spectrophotometer (Thermo Fischer Scientific, Waltham, MA). Libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego). Samples were pooled and genomic DNA was sequenced in Illumina MiSeq (Illumina, San Diego, CA, USA) with 2 150 bp paired-end reads.

Submitted by: Cornell University

Study: Klebsiella and Rahnella sp. isolates associated with ropy defect in milk

PRJNA907796 • SRP411052 • All experiments • All runs

show Abstracthide Abstract

Whole genome sequencing of Rahnella and Klebsiella sp. isolates associated with ropy defect of milk.

Sample:

SAMN31997487 • SRS15944798 • All experiments • All runs

Organism: Rahnella inusitata

Library:

Name: FSL A6-0213

Instrument: Illumina MiSeq

Strategy: WGS